Summary: There is no evidence to suggests that vitamin b boosts testosterone levels among humans, however studies on rats have shown positive results. I certainly would not require either of there ingredients in any testosterone boosting product nor would I take them individually to increase my t levels.

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Full Analysis:

There has been a significant surge in the public awareness about maintaining good health and general wellness. People are getting increasingvitamin b6 and b12 more willing to take supplements along with diet and multivitamins happen to be the most popular category. Vitamin B6, a water soluble vitamin is involved in glucose metabolism, lipid biosynthesis and production of important chemicals like neurotransmitters. Chemically vitamin B6 is called as pyridoxine and pyrodoxal 5 phosphate is its biologically active form. Complete vitamin B6 requirement of the body must be met by dietary intake as our body cannot synthesize this important vitamin. Vitamin B6 deficiency leads to health problems in cardiovascular system, skin, nervous system and immune functions. There are studies describing the effect of vitamin B6 supplements on reproductive system and hormones. Vitamin B6 is responsible for modulating the hormonal secretions from pituitary gland and steroid hormones. Scientific reports on impact of administering vitamin B6 in humans and rats are summarized here.


Vitamin B6 deficiency leads to low blood testosterone levels in rats

Study: Male rats were induced with vitamin B6 deficiency by feeding on vitamin deficient diet for 4 weeks. Vitamin B6 deficiency in test animals was ascertained by measuring the vitamin levels in blood. After 4 weeks of treatment, blood samples were collected for estimating testosterone levels and prostate tissues were collected by sacrificing the animals.

Result: Authors observed low serum testosterone levels in vitamin B6 depleted rats but the levels of luteinizing hormone remained unaltered. Prostate tissue analysis revealed high testosterone concentration and testosterone was found mainly localized in the nucleus of the cells. This localization was observed as the testosterone receptors were exclusively retained in the nucleus. Vitamin B6 is probably responsible for recycling the receptors (that bind testosterone) from nucleus to cytosol and lack of vitamin led to receptor localization in nucleus.

Conclusion: Vitamin B6 deficiency in male rats leads to reduced synthesis of testosterone.



Very high doses of vitamin B6 have no effect on testosterone levels in rats

Study : High doses ( 250 -1000 mg per kg body weight) of vitamin B6 were injected in male rats for 2 and 6 weeks. At the end of treatments, sperm counts, weight of testis, prostate gland , epididymus was taken and levels of key enzymes were assayed. Blood testosterone levels were quantified in all the treatment groups at the end of study.

Result: In all the treatment groups there was a decline in reproductive function parameters like sperm counts, weight of testis and prostate gland. However, the testosterone levels remained unchanged.

Conclusion: High dose of vitamin B6 does not alter the blood testosterone levels in rats.



Vitamin B6 important for brain development in infants

Study: A survey was undertaken on infants and pregnant women with vitamin B6 deficiency.

Result : Infants with deficiency show behavioral abnormality and compromised adaptive behavior. Pregnant women with low vitamin B6 levels gave birth to babies with lower than normal weight that eventually leads to developmental delays and poor cognitive abilities.

Conclusion: Vitamin B6 is critical for human brain development and pregnant ladies must be encouraged to consume diet rich in vitamin B6.



Vitamin B6 does not alter the levels of growth hormone and prolactin

Study: Tolis and co workers designed this study on 9 subjects with galactorrhea (milky discharge from nipples) but with normal and increased prolactin levels. All the subjects were administered vitamin B6 and post treatment the hormone levels were assessed.

Result : Vitamin B6 therapy did not impact growth hormone and prolactin levels. There was no improvement in galactorrhea or resumption of menses in any of the subjects after two months of vitamin B6 intake.

Conclusion: Vitamin B6 supplementation does not support in regulating the levels of hormones like prolactin.



Animal studies on vitamin B6 suggest its positive role in enhancing the testosterone levels. However, there are no human clinical reports directly indicating testosterone enhancing role of vitamin B6. There are scientifically validated studies linking the vital function of vitamin B6 in brain development, restoring normal thyroid function, facilitating normal absorption of nutrients and strengthening immunity in humans. Vitamin B6 requirement is sufficed by the dietary intake of cereals, eggs, fish, milk, carrots, potatoes and green leafy vegetables like spinach.

Another important vitamin of B complex group is Vitamin B12. Chemically is known as cobalamin and it is one of the most prominent vitamin consumed as a supplement. This vitamin is present in non vegetarian diet like meat and deficiency leads to pernicious anemia, neurological disorders and fatigue. Vitamin B12 helps in managing fertility, improving immunity and strength. Although there are no scientific studies directly implying the role of Vitamin B12 in increasing testosterone levels its role on fertility is documented.


Vitamin B12 improves semen parameters in sub fertile males

Study : Isoyama and co workers conducted this clinical investigation on 26 subfertile males with the hypothesis that Vitamin B12 supplementation for 4 to 24 weeks at a concentration of 1500 micrograms per day can improve semen parameters. At the end of study total sperm count, motility, morphology was recorded. Levels of hormones like luteinizing hormone, follicle stimulating hormone and testosterone were quantified in serum.

Result: Authors observed an improvement in semen parameters like sperm count, motility and morphology. The levels of testosterone, luteinizing hormone and follicle stimulating hormone remain unchanged.

Conclusion: Vitamin B12 helps in restoring male fertility but has no effect on testosterone levels.

Source :


Vitamin B6 and B12 supplement support important physiological functions in body including critical role in fertility and cognitive development. There is no concrete scientific evidence emanating from human clinical studies to support a direct role of these vitamins on testosterone levels. Studies on rats have shown an upward shift in testosterone levels on vitamin B6 treatment. These supplements are useful in maintaining general good health for individuals with history of certain chronic diseases that are linked to malabsorption of vitamins. Vitamin B complex supplements are also advised for pregnant ladies as it supports in fetal brain development.

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